pLOT Version 1.0.10ab
pLOT v1.0.10b is now up. In addition to a few possible bug fixes, you can now specify enzymes by the number of cuts and position in the sequence. An updated Restriction enzyme file has also been included that removes a couple double entries. Note that currently, the filtered enzyme selection does not save to the save file. This will be added shortly. Filters do not take into account the blunt/staggered cut, etc... parameters.
Example, using the pX330 vector sequence
Plasmid with no enzymes specified
Site Frequency tab in the restriction enzyme window.
In the above window, enzymes are selected if they cut only once in the sequence (first filter) but not in the first 1000bp (second filter). Note the number of enzymes matching the criteria is shown in parentheses below the tabs.
Add the filter as a new entry
Modify the currently selected filter
After selection of single cutting enzymes that do not cut in the first 1kb.
pLOT 1.0.10b Download Links
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